ROSA T-2 and HT-2 Quantitative Test

T-2 toxin, and its metabolite HT-2 toxin, may be found in cereal grains, particularly in oats. These mycotoxins are produced by Fusarium, which grows optimally in moist, cool conditions. Research is ongoing to evaluate the health effects of these toxins on human and animal health; however, researchers have shown that T-2 and HT-2 toxins are a concern for swine and poultry.

The T-2/HT-2 test enables grain and feed producers to quantitatively detect T-2 and HT-2 toxins. The Rapid One Step Assay is a lateral flow test that is read in the Charm EZ-M system or the ROSA-M Reader. Samples are extracted with 70% methanol.



The T-2/HT-2 test has been validated by Charm for the following additional commodities:
  • Barley
  • Corn
  • Corn Gluten Meal
  • Oat Groats
  • Sorghum
  • Soybean Meal
  • Wheat
  • Wheat Flour


  • Validated for eight commodities
  • Quantitation range is 25 to 200 ppb for the first dilution, 100 to 2000 ppb for the second dilution
  • Quantitative results in 10 minutes
  • Extract sample with 70% methanol
  • Uses same equipment as other ROSA grain tests


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