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Fast Phage MPN >> Available in kits of 24 and 25 testsColiphage are bacteriophage viruses that infect E. coli and coliform bacteria. They are indicators for enteric viruses analogous to using E. coli as indicators for pathogenic bacteria. In 2006, EPA added coliphage as an equivalent fecal indicator to E. coli and enterococci in the Ground Water Rule. Current bacteriological tests take 18 to 96 hours to determine a negative and current EPA methods for coliphage take 36 to 60 hours for result. Fast Phage MPN provides rapid 6-hour quantitatition of somatic coliphage that is useful as a fecal pollution index.  Charm offers two version of the Fast Phage MPN test. One for volumes up to 4 ml that uses a TEMPO® MPN card and one for volumes up to 100 ml that can use a variety of MPN techniques such as 10 tube or Quanti-Tray®. Both Fast Phage MPN methods give same day results. Results are comparable to overnight plaque determination such as Method 1602. Fast Phage MPN is a useful tool for source water and distribution systems as well as for industry. For example, it can be used to test irrigation waters and flume waters in fresh cut produce.
Fast Phage: A Rapid Modification of EPA Method 1601 EPA Method 1601 is a specified coliphage method in the Ground Water Rule. Fast Phage’s 16-hour plaque result is equivalent to Method 1601. Additional benefits of the Fast Phage method include: • Convenient ready-to-use reagents • Accelerated enrichment time • Rapid fluorescent prediction Method modifications may be implemented as equivalent to Method 1601 following intra-lab validation that demonstrate detection criteria specified in Performance Based Method System EPA 1601 are met.
TEMPO is a registered trademark of bioMérieux SA, Marcy-l'Étoile, France. Quanti-Tray is a registered trademark of IDEXX Laboratories, Inc., Westbrook, Maine |
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