Charm Alkaline Phosphatase Tests
Alkaline phosphatase is an enzyme naturally present in raw milk. It is an excellent indicator to verify completeness of proper milk pasteurization. Alkaline and acid phosphatase enzymes are also marker enzymes for endpoint cooking determination in further-processed/cooked meats. Charm Alkaline Phosphatase Tests for dairy and meat measure phosphatase activity via a selective substrate whereby the hydrolyzed product from the enzyme reaction generates a luminescence signal which is then interpreted by a Charm luminometer.
Click on a tab below to find out more about each test.
PasLite
Charm’s PasLite Test is the leading alkaline phosphatase test employed by the dairy industry worldwide. PasLite delivers accurate, precise and economical detection for alkaline phosphatase in under four minutes. No reagent preparation or warm-up time is required, and multiple samples can be run simultaneously. PasLite is an excellent fit for dairy HACCP as it determines pasteurization problems long before they reach a level of critical concern. The PasLite Test is a chemiluminescence-based EPAS method that can be run on most Charm luminometer systems, including the Charm novaLUM.
PASLITE AT A GLANCE
• < 4 minute assay time
• IDF/ISO approved as an international standard method
(ISO 22160 /IDF 209) for alkaline phosphatase in milk and
dairy products
• NCIMS/FDA approved method for Grade A dairy testing
• Read-out: mU/liter
• Limit of Detection: 20 mU/L
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F-AP Fast Alkaline Phosphatase Test
Charm F-AP Test is the fastest available phosphatase method to verify the completeness of pasteurization in milk products. The simple one-step procedure requires no reagent preparation and delivers results in just 45 seconds for most products.
F-AP AT A GLANCE
• NCIMS/FDA approved method for Grade A dairy testing
• Results for most products in just 45 seconds!
• Test: Milks, Creams, Chocolate Milk, and more
• No reagents or incubation required
• Limit of Detection: 20 mU/L
• Approved by the New Zealand Ministry of Agriculture and Forestry
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CHEF Test
Charm CHEF (Cooking Heat EFficiency) Test is the first single-use, post-process indicator of compliance with the food time-temperature profile. Designed to quickly detect undercooked meat in cooked products, the CHEF Test measures phosphatase activity as an indication of meat "doneness".
CHEF TEST AT A GLANCE
• 5-minutes assay
• No enrichment or culture time
• Enables real-time corrective action and quality assurance
• Detect as little at 0.01% raw product
• Applications: Beef, Poultry, Pork, Seafood
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