UD = Un-determined
ND = Procedure not developed

NOTES:

1. Preparation dilution 1 part sample to 3 parts buffer. Alternative control point procedure may be used to enhance sensitivity 2 fold.
2. Dilution range 1 part sample to 100-150 parts buffer to predict tissue action levels. Minimal allowable dilution 1:4 will enhance sensitivity 25 fold.
3. Dilution 1 part sample to 20 parts buffer to predict tissue action levels. Minimal allowable dilution 1:4 will enhance sensitivity 5 fold.
4. Dilution 1 part sample into 2 parts extract, 1 part extract to 25 parts buffer.
5. Dilution 1 part to 4 parts for beta-lactams, macrolides, streptomycins; 1 part to 10 parts for Sulfa Drugs and Tetracyclines; and 1part to 20 parts for Amphenicols.
6. Aminoglycoside detection is divided into two separate tests, streptomycin type aminoglycosides and neomycin type aminoglycosides. For milk and grain, gentamicin is detected by both tests while streptomycins are not detected by neomycin type and neomycin is not detected by streptomycin type. Kanamycin and amikacin are cross reactant with the neomycin type aminoglycoside test. For tissue, gentamicin is detected by the test for neomycin type