|
CHARM II Tetracycline Tests |
|
|
| Recommended Screening / Detection Levels |
|
Tetracyclines
|
Tissue1
ppb
|
Egg1
ppb
|
Urine2
ppb
|
Serum3
ppb
|
Feed4
ppb
|
Grain5
ppb
|
Honey5
ppb
|
Milk6
ppb
|
|
Tetracycline
|
20
|
20
|
600
|
40
|
100
|
100
|
4
|
4
|
|
Chlortetracycline
|
100
|
100
|
3000
|
200
|
800
|
800
|
20
|
28
|
|
Oxytetracycline
|
100
|
100
|
3000
|
200
|
800
|
800
|
20
|
19
|
|
Demeclocycine
|
UD
|
UD
|
UD
|
UD
|
UD
|
UD
|
UD
|
30
|
|
Doxycycline
|
UD
|
UD
|
UD
|
UD
|
UD
|
UD
|
UD
|
30
|
|
Methacycline
|
UD
|
UD
|
UD
|
UD
|
UD
|
UD
|
UD
|
30
|
|
Minocycline
|
UD
|
UD
|
UD
|
UD
|
UD
|
UD
|
UD
|
600
|
|
Rolitetracycline
|
UD
|
UD
|
UD
|
UD
|
UD
|
UD
|
UD
|
10
|
UD = Un-determined
ND = Procedure not developed
NOTES:
- Preparation dilution 1 part sample to 3 parts buffer. Alternative control point procedure may be used to enhance sensitivity 2 fold.
- Dilution range 1 part sample to 100-150 parts buffer to predict tissue action levels. Minimal allowable dilution 1:4 will enhance sensitivity 25 fold.
- Dilution 1 part sample to 20 parts buffer to predict tissue action levels. Minimal allowable dilution 1:4 will enhance sensitivity 5 fold.
- Dilution 1 part sample into 2 parts extract, 1 part extract to 25 parts buffer.
- Dilution 1 part to 4 parts for beta-lactams, macrolides, streptomycins; 1 part to 10 parts for Sulfa Drugs and Tetracyclines; and 1part to 20 parts for Amphenicols.
- Sensitivities listed are testing milk undiluted. For US safe levels a dilution of 1 part to 9 parts is recommended to screen at 300ppb total tetracyclines.
|
Antibiotic Tests 
